Description poste
We are seeking a highly skilled, entrepreneurial-minded CHO cell line development expert. You will be responsible for the execution of feasibility studies for industry partners, test different genetic integration strategies in CHO cells, and optimise growth conditions. The ideal candidate will be a creative thinker who is not afraid to think outside the box, with a strong industrial grounding, and excellent leadership and communication skills.
Roles and responsibilities:
• Be the driving force behind the development of stable mammalian cell lines expressing key therapeutic proteins of interest (both transiently and stably)
• Lead the optimisation and development of CHO cell medium for engineered cell lines, coordinating with third parties where required
• Adapt CHO cells to serum-free and suspension growth
• Explore and optimise gene expression in both CHO and HEK cells (testing different promoters, UTR’s, etc)
• Optimise & scale up growth of different CHO cell lines
• Quality reporting of cell banks
• Contribute to set up and development of in house analytical studies
• Take on managerial responsibilities as the team grows, contributing to growth and recruitment of a fast-growing R&D team
Description profil
Skills and requirements:
• Minimum 3 years relevant industry experience working with cell line development
• PhD in cell and molecular biology, biochemistry, biological engineering, or other life science discipline (exceptional Masters students with extensive industry experience will be considered) • Experience in suspension CHO/HEK cell line development; Vector design/Cloning; Stable and transient transfections; Single cell cloning; Clone selection (ELISA/Octet); Research Cell Banking (RCB) generation; Stability Assessment Studies; Genetic characterization methods (qPCR, whole genome sequencing)
• Experience leading projects or project sub teams and/or managing contract research relationships
• Excellent planning and organisation skills
• Team player who can deliver projects in a timely manner, yet work independently when needed
• Someone who wants to push the boundaries of scientific knowledge and tackle things they have never done before (or no one has!)
• A creative thinker with an entrepreneurial, problem-solving mindset
• Fluency in English
Bonus skills:
• Knowledgeable about GMP and regulatory requirements
• Postdoctoral studies
• Experience working with glycoproteins and/or the protein secretory pathway
• Mass spectrometry experience
• Patent inventor
• Experience working with plant and yeast recombinant expression systems
• French language
Poste proposé
Intitulé du poste proposé
Kyron.bio – Senior Scientist – CHO cell line development
Poste basé à Paris
Secteur d’activité : Biotechnology
Nature du contrat : CDI
Description entreprise
kyron.bio has made giant leaps in drug discovery over the past 5 years, however the bioproduction industry cannot keep up. kyron.bio is an early stage start-up with a mission to revolutionise therapeutic protein production. Our Next Generation Bioproduction Platform will boost both the effectiveness and the yield of proteins produced in mammalian cells. We are doing this by harnessing highly efficient processes from diverse organisms to genetically engineer the drug production platform of the future. We are the first company to use this approach and have a patent pending.Our vision is to generate the drug production platform of the future that will allow life-saving treatments to reach those that are most in need. We would love for you to join us on this mission.
